Fig. 1.

Schematic overview of THS-seq. High efficiency tagmentation is performed on gently lysed cells, followed by in vitro transcription, and custom RNA-seq to generate barcoded sequencing libraries. The following colors depict the following segments: light gray segments are Tn5 mosaic ends sequences, green segments are T7 promoter sequences, dark red segments are read primer sequences, dark blue segments are genomic DNA, light blue segments are cDNA sequences, purple segments are 3’ adaptor sequences, orange and navy blue segments are Illumina adaptor sequences, and yellow circles are barcodes