Fig. 4.

Protein-synthesis gene expression in placental tissues of pregnant, Walker tumour-bearing rats and ascitic fluid-injected rats fed either a control or leucine-rich diet. Mammalian target of rapamycin (mTOR) (a), p70-S6 kinase (p70S6K1) (b), eukaryotic initiation factors eIF4E (c) and eIF4G (d), eukaryotic translation initiation factor 4 binding protein 1 (4EBP1) (e), eIF5 (f) and eIF2a (g) transcript levels in placental tissue quantified by real-time PCR. Gene expression corrected by GAPDH as internal control. For additional details, see the Materials and Methods section. The results are expressed as the mean and standard error. Legend: Control (C), Leucine (L), Walker tumour-bearing rats (W), tumour-bearing rats fed a leucine-rich diet (WL), ascitic fluid-injected rats (A) and ascitic fluid-injected rats fed a leucine-rich diet (LA). * P < 0.05 accounted by tumour effect, two-way ANOVA with Bonferroni testing, n = 10