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. 2016 Feb 5;15:63. doi: 10.1186/s12936-016-1109-9

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© Sastu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PCR–RFLP of the pvdhfr gene. PCR–RFLP product of VDNF 57/VDF NR with BsrGI showed no cleavage of the 472 bp indicating wild type Phe58 and mutation 57Leu (a). Reaction with PvuII showed no cleavage (472 bp) indicating mutation 117Thr/Asn (b). Reaction with BstN1 cleaved the 472 bp to 258 and 215 bp indicating mutation 117Thr (c). Reaction with Bsr1 showed no cleavage indicating wild type Ser118 and mutation 117Thr (d). a, b, c and d: Lane L DNA ladder of Agilent DNA 1000 Kit (Agilent Technologies, Molecular Probes Inc, USA), Lanes 1–12 PCR–RFLP products of samples