Fig. 5.

PCR-RFLP of the pvdhps gene. PCR-RFLP of VDHPSNF/VDHPSNR with MspI cleaved the 703 bp fragment into 655 and 48 bp indicating mutation 383Gly (a). PCR–RFLP of VDHPS553OF/VDHPSNR with MscI digest the 170 bp to 143 and 28 bp indicating wild type Ala553 (b). a and b: Lane L DNA ladder of Agilent DNA 1000 Kit (Agilent Technologies, Molecular Probes Inc, USA). a: Lanes 1, 3, 5, 7, 9, and 11 PCR products of VDHPSNF/VDHPSNR, Lanes 2, 4, 6, 8, 10, and 12 digestion reaction with MspI. b: Lanes 1, 3, 5, 7, 9, and 11 PCR products of VDHPS553OF/VDHPSNR, Lanes 2, 4, 6, 8, 10, and 12 digestion reaction with MscI