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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Leuk Lymphoma. 2015 Oct 5;56(12):3401–3406. doi: 10.3109/10428194.2015.1023799

Figure 2.

Figure 2

Figure 2

TNF-induced expression of NFκB reporter constructs. HeLa cells were transiently transfected with expression plasmids for MnSOD2-luciferase (Panel A) or p65-HIV-luciferase (Panel B) along with Renilla luciferase as transfection control and NPM-RAR-pSG5 or pSG5 control. 48 hours after transfection 20 ng/ml TNF was added to the cultures. Lysates were prepared at 72 hours, and luciferase activity normalized for Renilla expression. The ratio of normalized luciferase activity induced by TNF compared to vehicle control was determined. Shown are the mean and standard error for seven (Panel A) and three (Panel B) independent experiments.