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TNF-induced JNK activation. Panel A. U937 cells or three NPM-RAR-stably expressing clones were treated with 10 ng/ml TNF or vehicle control for 3 minutes before cells were lysed and assayed for activated JNK kinase activity with SAPK/JNK Kinase Assay Kit. Panel B. HeLa cells or two NPM-RAR-stably expressing clones were treated with 10 ng/ml or vehicle control for 5 minutes before cells were lysed and assayed for activated JNK kinase activity with SAPK/JNK Kinase Assay Kit.
