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. Author manuscript; available in PMC: 2016 Dec 1.

Published in final edited form as: Leuk Lymphoma. 2015 Oct 5;56(12):3401–3406. doi: 10.3109/10428194.2015.1023799

Nuclear/cytoplasmic distribution of NPM-RAR and TRADD. Panel A. Nuclear and cytoplasmic extracts were prepared from HeLa cells or stably expressing NPM-RAR expressing clones, and run on SDS-PAGE gels. Immunoblots were probed with antibodies against RARA (which recognize NPM-RAR as well as wild-type RARA), lamin and HSP90. Panel B. HEK293 cells were transiently transfected with expression plasmids for NPM-RAR and V5-TRADD. 48 hours after transfection nuclear and cytoplasmic extracts were prepared and run on SDS-PAGE gels. Immunoblots were probed with antibodies against RAR, V5, lamin and HSP90.

Nuclear/cytoplasmic distribution of NPM-RAR and TRADD. Panel A. Nuclear and cytoplasmic extracts were prepared from HeLa cells or stably expressing NPM-RAR expressing clones, and run on SDS-PAGE gels. Immunoblots were probed with antibodies against RARA (which recognize NPM-RAR as well as wild-type RARA), lamin and HSP90. Panel B. HEK293 cells were transiently transfected with expression plasmids for NPM-RAR and V5-TRADD. 48 hours after transfection nuclear and cytoplasmic extracts were prepared and run on SDS-PAGE gels. Immunoblots were probed with antibodies against RAR, V5, lamin and HSP90.