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. 2016 Feb 5;9:73. doi: 10.1186/s13071-016-1355-2

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© Martínez-Valladares and Rojo-Vázquez. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Detection of the infection in experimentally infected sheep. a LAMP reaction after adding SYBR Green I; b Standard PCR with the primers F3 and B3 of the LAMP assay. P: positive control; N: negative control; 1–9: pooled samples of sheep on day 0 of the infection (1) and between 1 and 8 weeks post infection (2–9); M: marker