Table 2.
Comparison of hepatic fatty acid composition in control and TTP-treated mice
| Diet groups | ||||
|---|---|---|---|---|
| Fatty acida (wt %) | Control | 0.3 % TTP | 0.6 % TTP | 0.9 % TTP |
| Σ SFA | 32.0 ± 2.8 | 31.1 ± 1.5 | 29.3 ± 1.8* | 29.0 ± 1.7** |
| C12:0 | 0.01 ± 0.003 | 0.05 ± 0.02* | 0.05 ± 0.02** | 0.05 ± 0.02** |
| C14:0 | 0.31 ± 0.09 | 0.53 ± 0.14** | 0.69 ± 0.19*** | 0.71 ± 0.17*** |
| C18:0 | 10.3 ± 2.4 | 8.5 ± 1.4 | 6.6 ± 2.1** | 6.7 ± 1.0** |
| C20:0 | 0.23 ± 0.05 | 0.14 ± 0.05*** | 0.07 ± 0.03*** | 0.06 ± 0.01*** |
| C22:0 | 0.29 ± 0.07 | 0.21 ± 0.06* | 0.11 ± 0.07*** | 0.10 ± 0.04*** |
| C24:0 | 0.22 ± 0.06 | 0.13 ± 0.02** | 0.10 ± 0.05*** | 0.09 ± 0.04*** |
| Σ MUFAs | 27.0 ± 5.1 | 29.7 ± 3.7 | 39.6 ± 8.8** | 41.5 ± 5.1*** |
| C16:1n-7 | 3.0 ± 0.9 | 3.6 ± 0.3 | 4.7 ± 1.5* | 4.9 ± 1.3* |
| C16:1n-9 | 0.38 ± 0.14 | 0.58 ± 0.06* | 0.82 ± 0.21*** | 0.93 ± 0.18*** |
| C18:1n-9 | 19.5 ± 5.1 | 22.6 ± 2.2 | 29.9 ± 6.4*** | 31.8 ± 4.1*** |
| C20:1n-7 | 0.18 ± 0.07 | 0.09 ± 0.02** | 0.09 ± 0.05** | 0.06 ± 0.03*** |
| C22:1n-7 | 0.05 ± 0.01 | 0.02 ± 0.01*** | 0.01 ± 0.01*** | 0.01 ± 0.01*** |
| C24:1n-9 | 0.27 ± 0.06 | 0.19 ± 0.03* | 0.16 ± 0.06*** | 0.14 ± 0.04*** |
| Σ n-6 PUFAs | 30.6 ± 3.7 | 27.8 ± 1.5 | 21.9 ± 6.1*** | 20.7 ± 3.0*** |
| C20:2n-6 | 0.25 ± 0.07 | 0.20 ± 0.03 | 0.18 ± 0.05* | 0.17 ± 0.03* |
| C20:3n-6 | 1.7 ± 0.2 | 1.2 ± 0.1*** | 0.9 ± 0.4*** | 1.0 ± 0.2*** |
| C20:4n-6 | 13.4 ± 2.1 | 10.5 ± 1.5** | 6.8 ± 2.6*** | 6.8 ± 1.2*** |
| C22:5n-6 | 0.42 ± 0.29 | 0.18 ± 0.04* | 0.12 ± 0.04** | 0.13 ± 0.03** |
| Σ n-3 PUFAs | 10.0 ± 0.9 | 11.1 ± 1.4 | 8.9 ± 3.1 | 8.5 ± 1.6 |
| C18:3n-3 | 0.26 ± 0.06 | 0.51 ± 0.12* | 0.48 ± 0.24* | 0.51 ± 0.20* |
| C18:4n-3 | 0.02 ± 0.01 | 0.06 ± 0.02 | 0.07 ± 0.05* | 0.07 ± 0.05* |
| C20:5n-3 | 0.39 ± 0.09 | 0.46 ± 0.07 | 0.43 ± 0.20 | 0.40 ± 0.15 |
| C22:5n-3 | 0.28 ± 0.06 | 0.44 ± 0.04* | 0.40 ± 0.20 | 0.39 ± 0.16 |
| C22:6n-3 | 8.9 ± 0.8 | 9.5 ± 1.5 | 7.4 ± 2.7 | 7.0 ± 1.2 |
| Σ n-3 PUFA/Σ n-6 PUFAb | 0.33 ± 0.02 | 0.40 ± 0.04** | 0.40 ± 0.06** | 0.41 ± 0.04** |
Abbreviations: SFAs saturated fatty acids; MUFAs monounsaturated fatty acids; PUFAs polyunsaturated fatty acids; TTP tetradecylthiopropionic acid
aMeans with standard deviation are shown (n = 7–8), and results were analyzed by one-way ANOVA with Dunnett’s post hoc test (P* < 0.05, P** < 0.01, P*** < 0.001)
bn-3 PUFA/n-6 PUFA calculated as: (18:4n-3 + 18:3n-3 + 20:3n-3 + 20:4n-3 + 21:5n-3 + 22:6n-3 + 22:5n-3)/(18:3n-6 + 18:2n-6 + 20:4n-6 + 20:3n-6 + 20:2n-6 + 22:5n-6 + 22:4n-6 + 22:2n-6)