Fig. 2.

Binding of native EgAgB to THP-1 derived monocytes and macrophages. Binding of native EgAgB to THP-1 monocytes and macrophages was evaluated using biotinylated EgAgB or biotinylated OVA as control (20–350 μg/ml). In (a) and (b) Binding indexes of OVA (grey) or native EgAgB (red) for monocytes and macrophages, respectively; data are expressed as mean values ± SEM of triplicates. Asterisks (*) denote significant differences in binding indexes of native EgAgB with respect to the control (two-way ANOVA followed by Bonferroni’s post-test, p < 0.05). (c), (d) Titration curves obtained for OVA (▲) or EgAgB (●) binding to monocytes or macrophages, respectively. Several fitting models of Graph Prism software were tested and the “binding saturation with one side-specific binding” model showed the best R² (0.992). The solid line corresponds to the theoretical binding curve obtained for each protein. One representative experiment of three is shown in each panel