Table 1.
Identification of EgAgB8 apolipoproteins in Infertile-fQS by LC-MS/MS
| Apolipoprotein | Uniprot Accession Number | Molecular Mass (Da) | pI | Intensity (% coverage) | iBAQ | Unique peptides |
|---|---|---|---|---|---|---|
| EgAgB8/1 | Q5EKQ4 | 7589.9 | 8.31 | 29.7 (53.5) | 28.1 | DDGLTSTSR, DPLGQKVVDLLK, DPLGQKVVDLLKELEEVFQLLR, ELEEVFQLLR, ELEEVFQLLRK, VVDLLKELEEVFQLLR, VVDLLKELEEVFQLLRK, YFFERDPLGQK |
| Q9UA06a | 7555.9 | 8.31 | ||||
| EgAgB8/2 | Q5EKP1 | 7906.2 | 9.42 | 21.1 (37.7) | 19.5 | AHMGQVVK, AHMGQVVKKDFFRNDPLGQR, LVALGNDLTAICQK, NDPLGQR, NLVEEKDDDSK, YVKNLVEEK, YVKNLVEEKDDDSK |
| Q27275b | 8193.5 | 9.37 | ||||
| EgAgB8/3 | Q95NW6c | 7858.2 | 8.02 | 24.0 (33.9) | 22.4 | DDDDDEVTK, DVASVCEMVR, HFFQSDPLGK, HFFQSDPLGKK |
| A0A068X006–1 | 6712.8 | 6.78 | ||||
| EgAgB8/4 | Q6Q0H5 | 8353.66 | 6.20 | 24.1 (27.3) | 22.6 | DFFRSDPLGQR, DLLEEEEEEDDSK, DLTAICQK, YVKDLLEEEEEEDDSK |
| EgAgB8/5 | Q5EKP9 | 7657.07 | 9.56 | 21.0 (14.7) | 19.4 | EVASVCQMVR |
The following proteins (Uniprot accession numbers) generate identical mature products to that indicated with a superscript letter: a U6JQF4 and Q5S577, b Q5EKN4, C1KBK4, Q6Q0H3 and Q6Q0I3, c Q5EKQ8, Q5EKR1, Q5EKR3 and Q95W92
Molecular mass and isoelectric point (pI) of mature proteins were calculated using the “compute pI/MW” Expasy tool (http://web.expasy.org/compute_pi/)
Intensity values correspond to log2 of summed XIC (extracted ion current) of all isotope clusters associated to the corresponded protein and are given as median of quintuplicates. The iBAQ values were also log2-transformed and their relative standard deviation was ≤ 1.1 %
Coverage values correspond to the percentage of the protein sequence that is covered by the identified peptides