Fig. 2.

Banding patterns of PrP^res in the brain and spleen tissue of ovine PrP mice infected with MM2-sCJD prions. (a) PrP^res banding patterns in the brain and spleen tissue of ovine PrP mice (tg338 line) over two serial passages of MM2-sCJD (MM2). Note the different patterns in brain and spleen, which were designated T2^Ov (red arrow) and T1^Ov (blue arrow), respectively. These patterns can be compared with those observed after inoculation of 127S scrapie prions, a strain with a 21 kDa signature in brain and spleen [34]. (b) Immunoblots showing PrP^res pattern in the brain and spleen of tg338 mice over serial passage of uncloned MM2-sCJD (MM2) and cloned (Cl.) MM2-sCJD prions. Note the low levels of T2^Ov PrP^res in the spleens of tg338 mice inoculated with cloned MM2-sCJD prions, which are only visible on overexposed gels. The equivalent of 1 and 2 mg of tg338 brain and spleen tissue was loaded on the SDS-PAGE gels. The blots were probed with Sha31 antibody