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. 2015 Aug 12;5(4):e1080787. doi: 10.1080/21597081.2015.1080787

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — (A) PlyPalA inhibition by the metal cation chelator EDTA. After adding P. larvae and before adding lysin, EDTA was added to various concentrations as ratios of the known concentration of Mg²⁺ (5.0 mM). Error bars represent standard deviations (n = 3). (B) PlyPalA activity with Mn²⁺, Co²⁺, and Ca²⁺ cofactors. Cations were added to a concentration of 5.0 mM. The (-) control represents lysis with no cation added. Readings were taken once per minute for 30 mins. Error bars represent standard deviations (n = 3).