Table 1.
P. larvae strain | % Loss in OD600 | ERIC Genotype |
---|---|---|
NRRL B-3650 | 84.9% ± 0.6% | I |
NRRL B-3554 | 78.0% ± 0.9% | I |
ATCC 25748 | 72.8% ± 2.0% | I |
NRRL B-2605 | 72.1% ± 1.2% | I |
ATCC 25747 | 62.7% ± 1.4% | I |
2188 | 57.2% ± 2.6% | I |
ATCC 25367 | 49.4% ± 1.6% | IV |
2231 | 42.4% ± 0.5% | I |
ATCC 3688 | 23.3% ± 2.2% | III/IV |
ATCC 25368 | 22.9% ± 3.3% | IV |
ATCC 49843 | 12.2% ± 2.1% | IV |
E. coli (-) | -1.6% ± 3.1% | N/A |
aPlyPalA is most effective against P. larvae strains corresponding to ERIC genotype I. Standard deviations were calculated from the ratio of OD600 of lysin-treated wells versus buffer treated wells (in triplicate). E. coli was used as a negative control. ERIC genotypes for NRRL B-2605 and ATCC 49843 were taken from a previous study17 and determined experimentally by ERIC PCR for other strains (data not shown).