Table 2.
Species and genus specificity of PlyPalAb
| Bacterial Genus/Species | Strain | % Loss in OD600 |
|---|---|---|
| Paenibacillus larvae (+) | NRRL B-3650 | 80.0% ± 0.9% |
| Paenibacillus polymyxa | DSM 36 | 26.2% ± 3.4% |
| Paenibacillus lentimorbus | ATCC 14707 | 20.0% ± 2.7% |
| Gluconobacter cerinus | ATCC 19441 | 10.4% ± 5.4% |
| Listeria innocua | ATCC 51742 | 8.0% ± 4.8% |
| Paenibacillus alvei | ATCC 6344 | 7.9% ± 6.2% |
| Staphylococcus aureus | ATCC 12600 | 8.2% ± 1.2% |
| Fructobacillus fructosus | ATCC 13162 | 6.7% ± 5.5% |
| Lactobacillus brevis | NRRL-1834 | 5.6% ± 2.8% |
| Bacillus subtilis | YB955 | 6.2% ± 6.9% |
| Bacillus megaterium | ATCC 12872 | 5.5% ± 1.8% |
| Paenibacillus popilliae | ATCC 14706 | 5.4% ± 7.2% |
| Lactobacillus gasseri | ATCC 3323 | 2.5% ± 1.4% |
| Bifidobacterium longum | ATCC BAA-999 | 1.3% ± 3.2% |
| E. coli (-) | 11303B | 0.58% ± 2.4% |
bPlyPalA is highly species-specific although it does exhibit some lysis against other Paenibacillus spp. Standard deviations were calculated from the ratio of OD600 of lysin-treated wells versus buffer treated wells in triplicate. E. coli was used as a negative control. P. larvae 3650 was used as a positive control.