Table 4.

ROS and 4-HNE levels and MITO complexes I, II, III, IV, and V abundances in LV myocardium of normal dogs (n=6), untreated heart failure control dogs (HF-CON) (n=7) and elamipretide-treated heart failure dogs (HF+ELA) (n=7).

	Normal	HF-CON	HF+ELA
LV Tissue ROS (RLUx10⁴/μg protein)	0.77±0.04	1.45±0.18^*	0.90±0.06^*^†
4-HNE adducts (ng/mg protein)	185±21	399±35^*	252±18^*^†
Complex-I subunit (du)	0.51±0.02	0.15±0.01^*	0.29±0.03^*^†
Complex-II subunit (du)	0.45±0.06	0.14±0.01^*	0.34±0.02^*^†
Complex-III subunit (du)	0.40±0.07	0.16±0.02^*	0.27±0.03^*
Complex-IV subunit (du)	0.61±0.04	0.26±0.05^*	0.44±0.03^*^†
Complex-V subunit (du)	4.97±0.32	2.12±0.37^*	2.81±0.50^*

ROS=reactive oxygen species; 4-HNE=4-hydroxynonenal. All data are Mean±SEM.

p<0.05 vs. Normal;

^†

p<0.05 vs. HF-CON.