Table 2. ESMS determination of α and β chain molecular weight.
α chaina 15,125 D | β chaina 15,867 D | α chain modifieda 15,252 Db | β chain modifieda 15,992 Dc | |
---|---|---|---|---|
|
||||
Hb A | 100 | 100 | — | — |
NEM-L | 88 ± 7 | 12 ± 16 | 12 ± 7 | 88 ± 17 |
NEM-H | 67 ± 8 | 0 | 33 ± 11 | 100 |
Percentage of chain present relative to total.
MW = 1 α chain + 1 NEM.
MW = 1 β chain + 1 NEM