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. 1969 Nov;22(6):710–717. doi: 10.1136/jcp.22.6.710

Use of activated charcoal in the radioimmunoassay of human growth hormone in plasma

H S Jacobs 1
PMCID: PMC474366  PMID: 5365346

Abstract

The use of activated charcoal in the radioimmunoassay of human growth hormone is described. The method permits the rapid separation of bound from free labelled human growth hormone in large batches and affords a quick method for screening iodination eluates. The necessity for an equality of protein concentration for the incubation and the separation procedure is emphasized. The method is simple, reliable and rapid, and the sensitivity obtained compares favourably with that of other methods of separation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G. E. A rapid method for checking the purity of radioiodinated human luteinizing hormone. Clin Chem. 1969 Mar;15(3):230–236. [PubMed] [Google Scholar]
  2. Berson S. A., Yalow R. S. Iodoinsulin used to determine specific activity of iodine-131. Science. 1966 Apr 8;152(3719):205–207. doi: 10.1126/science.152.3719.205. [DOI] [PubMed] [Google Scholar]
  3. Boden G., Soeldner J. S. A sensitive double antibody radioimmunoassay for human growth hormone (HGH): levels of serum HGH following rapid tolbutamide infusion. Diabetologia. 1967 Oct;3(5):413–421. doi: 10.1007/BF01228076. [DOI] [PubMed] [Google Scholar]
  4. Donald R. A. Application of the coated charcoal separation method to the radio-immunoassay of plasma corticotrophin. J Endocrinol. 1968 Aug;41(4):499–508. doi: 10.1677/joe.0.0410499. [DOI] [PubMed] [Google Scholar]
  5. GOTTLIEBLAU K. S., WASSERMAN L. R., HERBERT V. RAPID CHARCOAL ASSAY FOR INTRINSIC FACTOR (IF), GASTRIC JUICE UNSATURATED B12 BINDING CAPACITY, ANTIBODY TO IF, AND SERUM UNSATURATED B12 BINDING CAPACITY. Blood. 1965 Jun;25:875–884. [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., KLOPPER A. ASSAY OF HUMAN GROWTH HORMONE IN PREGNANCY AT PARTURITION AND IN LACTATION. DETECTION OF A GROWTH-HORMONE-LIKE SUBSTANCE FROM THE PLACENTA. Br Med J. 1964 Jan 4;1(5374):22–24. doi: 10.1136/bmj.1.5374.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  8. Herbert V., Lau K. S., Gottlieb C. W., Bleicher S. J. Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab. 1965 Oct;25(10):1375–1384. doi: 10.1210/jcem-25-10-1375. [DOI] [PubMed] [Google Scholar]
  9. Lau K. S., Gottlieb C. W., Herbert V. Preliminary report on coated charcoal immunoassay of human chorionic "growth hormone-prolactin" and growth hormone. Proc Soc Exp Biol Med. 1966 Oct;123(1):126–131. doi: 10.3181/00379727-123-31420. [DOI] [PubMed] [Google Scholar]
  10. Odell W. D., Parlow A. F., Cargille C. M., Ross G. T. Radioimmunoassay for human follicle-stimulating hormone: physiological studies. J Clin Invest. 1968 Dec;47(12):2551–2562. doi: 10.1172/JCI105937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rosselin G., Assan R., Yalow R. S., Berson S. A. Separation of antibody-bound and unbound peptide hormones labelled with iodine-131 by talcum powder and precipitated silica. Nature. 1966 Oct 22;212(5060):355–357. doi: 10.1038/212355a0. [DOI] [PubMed] [Google Scholar]

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