Figure 6.

Cultured normal limbs and their response to JNK inhibitor. (A) Whole-mount in situ hybridization to detect Dlx2. Legend as in Figure 5. Red asterisks indicate ‘absence of signal’. (B) Whole-mount in situ hybridization to detect Shh expression as control of no off target effects of JNKi. (C) Real-time qPCR to quantify the expression of Dlx5, Wnt5a, Fgf8, RhoU and Wnt10b, in cultured WT hindlimbs (B) or forelimbs (C). In each case, the limbs were either treated with JNKi (patterned bars) or left untreated (open bars). The expression in the untreated limbs is normalized =1. Asterisks indicate the statistical significance.