Fig 5. KIF11 plays a role in cellular process formation.

(A) Nascent cellular process formation was monitored for 6 hours via time-lapse microscopy in the presence of vehicle (DMSO) or ispinesib (200 nM) in 08-387 TICs plated on GelTrex and enriched in interphase. Representative images of the two treatment groups are shown at the 6-hour time point. Cell bodies are masked in red and cell processes masked in yellow. Scale bars represent 10 μm. (B) Process length was measured over the time course. Error bars represent s.d., n > 500 cells/condition from 3 biological replicates, p < 0.0001, Two-way ANOVA with a Bonferroni post-test. (C) Representative images from a modified scratch wound assay used to drive formation of a leading cellular process using 08-387 TICs enriched in interphase. Just prior to wound formation, media was changed to that containing vehicle (DMSO) or ispinesib (200 nM). 6 hours later, cells were fixed and processed for immunofluorescence to βtubulin. (D) Anti-tubulin fluorescence signal from confocal images of the primary cell layer adjacent to the wound was quantified for both treatment groups and presented as signal over area. Horizontal bar represents the mean and error bars represent s.d. n > 40 cells/condition, p < 0.0001, unpaired t-test.