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. 2016 Jan 11;113(4):1032–1037. doi: 10.1073/pnas.1514586113

Fig. 1.

Fig. 1.

Association of nuclear TRAF3 with CREB in B cells. (A and B) CH12.LX or CH12.TRAF3−/− B cells were fractionated into nuclear and cytoplasmic lysates. CREB (A) and CBP (B) were immunoprecipitated from the cytoplasmic and nuclear fractions. Western blots were probed for indicated proteins. Data from one of three independent experiments are shown. (C and D) Primary B cells were isolated from spleens of littermate WT and B-Traf3−/− mice. (C) The 5 × 106 WT B cells were stimulated with 200 ng/mL of recombinant BAFF or 5 μg/mL of anti-mouse CD40 mAb for 20 h at 37 °C. Nuclear and cytoplasmic cell lysates were immunoblotted for TRAF3, with actin and YY1 serving as loading controls. Representative blots from one of three independent experiments are shown. (D) Splenic B cells were stained with anti-TRAF3 Ab and assayed with immunofluorescence confocal microscopy. Representative images from one of three independent experiments are shown.