Fig. 5.

TRAF3-mediated regulation of CREB in human B cells. (A) CREB was immunoprecipitated from lysates of human B cells and Western blots were probed for CREB and TRAF3. IgG Ab isotype control Ab is also shown. Data are representative of two independent experiments. (B) Human B cells were stained with anti-TRAF3 Ab and TRAF3 expression was assayed with immunofluorescence confocal microscopy. Isotype control is also shown. Representative images of one of three independent experiments are shown. (C) Human B cells were transfected with siRNA targeting TRAF3 (Left) or TRAF2 (Right) and control siRNA for 48 h. Whole cell lysates were immunoblotted for indicated proteins. Data are representative of three independent experiments.