Fig. S2.

Changes in CREB ubiquitination in the absence of TRAF3. (A) Ubiquitinated fractions recovered from cell lysates of WT and TRAF3^−/− B cells as described in Materials and Methods were blotted for CREB. Actin and CREB levels in input lysates are also shown. Data are representative of two independent experiments. (B) CREB was immunoprecipitated from lysates of WT and TRAF3^−/− mouse primary B cells. Samples were analyzed with Western blotting for K48 linkage-specific ubiquitination. Data are representative of two independent experiments.