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. 2016 Jan 11;113(4):984–989. doi: 10.1073/pnas.1508535113

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Fig. 2. — Aberrant mitosis/cytokinesis in mutant cells bearing Ptpn11 GOF mutations. Mitosis and cell division in living Ptpn11^+/+ and Ptpn11^D61G/D61G MEFs at passage 3–5 were monitored by time-lapse video microscopy. (A) Average times of mitosis (from nuclear membrane breakdown to completion of cell division) for Ptpn11^+/+ and Ptpn11^D61G/D61G MEFs. (B) The percentages of the cells with abnormal abscission and the percentages of the daughter cells with two nuclei due to failed cytokinesis were quantified. (C) Mitotic Ptpn11^+/+ and Ptpn11^D61G/D61G MEFs (passage 5) arrested by nocodazole were collected by shake-off and then released to regular culture medium. Eighty minutes later, cells were fixed and immunostained with anti–β-tubulin antibody and counterstained with DAPI. The percentages of the cells with binuclei or multinuclei in anaphase and telophase were quantified.