Fig. S9.

Important role of phosphorylation of Y¹⁶⁶ and Y⁴⁴⁵ for the activation of Plk1. Shp2 E76K cDNA and the control vector were transfected into HeLa cells. Shp2 E76K-overexpressing cells were then transfected with Plk1 shRNA targeting 3′UTR. These Plk1 knockdown cells were then transfected with WT GFP-Plk1, GFP-Plk1 mutants with Y166F or Y445F mutations (without 3′UTR), or the control vector. Anaphase/telophase cells with lagging chromosomes were counted. These experiments were performed three times, and more than 50 mitotic cells for each group were examined. Data shown are presented as mean ± SD of three independent experiments.