Fig. 2.
Knockdown or overexpression of β3GalT5 in MCF-7 and MDA-MB-231 cell culture reduced or increased the level of SSEA-3 on cell surface and stemness properties by FACS analysis. The expression of CSC markers and SSEA-3 in parental cells. The level of SSEA-3 in subpopulations CD44+CD24-/lo and ESAhi PROCRhi gated in overexpressed β3GalT5 group was also determined. (A) The expression of CD24, CD44, and SSEA-3 in MCF-7 with overexpression, knocked down of β3GalT5 or their corresponding vector control. (B) The expression of ESA, PROCR and SSEA-3 in MDA-MB-231 with overexpression, knockdown down of β3GalT5 or their corresponding vector control. All of experiments are the representative sample from triplicate.