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. 2016 Jan 11;113(4):1068–1073. doi: 10.1073/pnas.1524650113

Fig. 2.

Fig. 2.

Mutation of the IM motif of CaV2.1 channels impairs synaptic facilitation in paired-pulse stimuli. (A) Example paired EPP from NMJ synapses at a 15-ms ISI in WT (black) and IM-AA (red) preparations. (B) Mean amplitudes of EPPs in response to the first stimulus in WT (black; n = 11) and IM-AA (red; n = 20). (C) Threshold stimuli for evoking muscle contraction in WT (black; n = 9) and IM-AA (red; n = 13) mice. (D) Average quantal content in WT (black, n = 10) and IM-AA (red; n = 12) mice. (E) Percentage block of EPP amplitude by ω-Aga-IVA in WT (black; n = 3) and IM-AA (red; n = 3) mice, and by ω-conotoxin GVIA in WT (gray; n = 3) and IM-AA (pink; n = 3) mice. (F) PPR plotted as a function of ISI in WT (black; n = 16) and IM-AA (red; n = 23) mice. All recordings were made in the presence of 0.75 mM Ca2+, except for measurements of quantal content, which were done in the presence of 2 mM Ca2+. *P < 0.05; **P < 0.01; ***P < 0.001.