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. 2016 Jan 11;113(4):E430–E439. doi: 10.1073/pnas.1514375113

Fig. S3.

Fig. S3.

Altered G3PP expression in INS832/13 cells affects lipid and energy metabolism. (A and B) Fatty acid esterification to 1,3-DAG, lysophosphatidylinositol (LPI) and lysophosphatidylcholine (LPC). (A) RNAi knockdown of G3PP and (B) overexpression of hG3PP (hPGP). Fatty acid esterification was measured by using [1-14C]palmitate at 2 mM and 10 mM glucose. (C and D) Oxygen consumption, ATP production, and H+ leak. Respiratory measurements in transfected cells were made at 2 mM and 10 mM glucose using a Seahorse XF analyzer and ATP production and H+ leak were calculated. (C) RNAi knockdown of G3PP and (D) hG3PP overexpression (means ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001 vs. GFP or control siRNA groups; n = 12).