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. 2016 Jan 11;113(4):1026–1031. doi: 10.1073/pnas.1514511113

Fig. S4.

Fig. S4.

Impact of FGF21 overexpression on TCR repertoire. (A) The CD4 cells were sorted from spleen from WT and Fgf21 tg mice (14 mo old) to prepare cDNA that was used for TCR spectratyping. The CDR3 polymorphism analysis revealed that 14-mo-old Fgf21 tg mice do not displayed significant perturbation of TCR diversity. The 3D graph depicts perturbation in CD4 T cells. Each line crossing on the y axis of the landscape denotes change from splenic CD4 cells-specific CDR3 length or size (x axis) of a particular Vβ family (z axis). The perturbation in TCR diversity is shown as landscape surfaces, in which smooth (blue) landscapes show an unchanged TCR diversity. (B) The CD4 cells were sorted from spleen from WT and FGF21 tg mice (14 mo old) to prepare cDNA that was used for TCR spectratyping. TCR diversity of peripheral CD4 T cells was analyzed by measuring the distribution of lengths of the CDR3 of TCR. Representative TCR Vβ profile in aged WT mouse and polyclonal Gaussian distribution of CDR3 lengths in Fgf21tg mice. The data were generated using an ABI 3100 sequencer and analyzed using GeneMapper.