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. 2016 Feb 5;11(2):e0148454. doi: 10.1371/journal.pone.0148454

Fig 8. Effects of heparin on OSCC cell-derived exosome-induced proliferation, migration, and invasion of OSCC cells.

Fig 8

OSCC cell lines were treated with 10 μg/mL heparin only once at the beginning of the experiment (single administration group) or every 4 h (multiple administration group) in the presence or absence of 50 μg exosomes for 16 h (A), 24 h (B), 10 h (C), or 18 h (D). Cellular internalization of OSCC cell-derived exosomes into OSC-4 cells was analyzed by confocal maicroscopy (A). The viable (B), migrating (C), and invading (D) cell numbers were then determined by the MTT, wound healing, and invasion assay, respectively. The values are presented as the mean ± SD; n = 3 for each group. * p < 0.05 against control of OSCC cells, by Mann–Whitney's U-test.