Fig. 3.

Southern blot of eight RILs from the DV92 × G3116 mapping population. DNAs were digested with restriction enzyme Eco RV and hybridized with the complete barley Cbf3 cDNA as a probe. Arrows indicate the RFLP fragments with strong hybridization signal mapped at the XCbf3 locus. Arrowheads indicate two additional RFLP fragments that cosegregate with the previous ones. Arrows and arrowheads on the left indicate G3116 alleles and on the right DV92 alleles. Numbers on the right of the figure indicate additional RFLP fragments with low hybridization signal that were not mapped.