Table 1. Statistical analysis of assay performance.

# compounds screened	S:Na	S:Bb	SWc	Z’d	% nCVintere	% nCVintraf	% pCVinterg	% pCVintrah
3,200	34.67	4.98	4.47	0.55	9.78	5.46 ± 2.3	11.49	6.30 ± 1.2
12,800	27.94	5.81	5.55	0.58	8.65	2.51 ± 1.1	17.21	4.51 ± 4.6
12,800	44.08	5.7	4.67	0.57	10.02	2.06 ± 0.8	10.67	2.70 ± 1.5
3,520	29.59	5.51	5.48	0.58	8.67	3.28 ± 2.0	15.24	5.49 ± 2.6
7,680	46.51	6.13	5.27	0.6	9.47	4.61 ± 0.7	11.04	6.64 ± 3.1
Average ± SD	36.56 ± 8.4	5.63 ± 0.4	5.09 ± 0.5	0.58 ± 0.02	9.32 ± 0.6	3.58 ± 1.4	13.13 ± 2.9	5.13 ± 1.6

^aSignal to noise ratio is a measure of signal strength

^bSignal to background ratio

^cSignal Window is a measure in the difference in signal between negative and positive controls

^dZ-factor is a measurement of the performance of the assay under the defined conditions

^e,f Inter- and Intra-plate, respectively, variability for negative control.

^g,h Inter- and Intra-plate variability, respectively, for positive control.