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. 2016 Feb 5;11(2):e0148645. doi: 10.1371/journal.pone.0148645

Fig 3. Effect of EGCG on IL-1β-induced IL-8 expression in orbital fibroblasts.

Fig 3

Orbital fibroblasts were pre-treated with EGCG for 1 h, followed by treatment with IL-1β (10 ng/mL) for 24 h. (A) The level of IL-8 was determined using by ELISA (R&D Systems, Minneapolis, MN). Similar results were obtained in three independent experiments with orbital fibroblasts of passages (P) 9 and P10 from TAO patient #53 and P4 from TAO patient #55. (B) The IL-8 mRNA levels were determined by quantitative real time RT-PCR). *p < 0.05 compared with cells treated with the same concentration of IL-1β as calculated by one-way ANOVA. Similar results were obtained in four independent experiments with orbital fibroblasts of P7, P9, and P10 from TAO patient #53 and P4 from TAO patient #55. P, number of passages.