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. 2016 Feb 5;11(2):e0147919. doi: 10.1371/journal.pone.0147919

Fig 4. Neutralization of LPS-induced pro-inflammatory cytokine production by CATH-2 analogs.

Fig 4

IL-1β transcription in HD11 cells stimulated with S. minnesota LPS (50 ng/ml) and CATH-2 derived peptides (20 μM). Transcription was measured by real time QPCR analysis after 4 h of stimulation. Amino acid substitutions: W3, [F2W, F5W, F12W]; W2, [F5W, F12W]; W, [F12W]; Y3, [F2Y, F5Y, F12Y]; Y2, [F5Y, F12Y]; Y, [F12Y]. a LPS-induced IL-1β transcription in HD11 cells was blocked by full-length peptide and N-terminally truncated C(1–21) analogs up to 18 amino acid residues. b Phe/Trp substitution improved the capacity to neutralize LPS-induced IL-1β transcription of peptides C(1–21) and C(4–21) and introduced LPS-neutralizing capacity in formerly inactive peptides C(7–21) and C(10–21). c Phe/Tyr substitution of active peptides C(1–21) and C(4–21) abrogated neutralization of LPS-induced IL-1β expression. Data from 3 to 4 independent experiments; means ± SEM. * p<0.05, ** p<0.01, *** p<0.001.