Table 3. Effect of aromatic amino acid substitution on HD11 cytokine expression levels.
| 4h stimulation | 24 h stimulation | |||||||
|---|---|---|---|---|---|---|---|---|
| IL-1β | CXCLi2 | MCP-3 | CCLi4 | IL-1β | CXCLi2 | MCP-3 | CCLi4 | |
| Medium | 1.0±0.1 | 1.0±0.1 | 1.0±0.2 | 1.0±0.3 | 1.0±0.1 | 1.0±0.1 | 1.0±0.1 | 1.0±0.0 |
| C(1–21) | 3.4±0.2 | 4.8±1.8 | 6.7±3.4 | 2.7±1.8 | 1.9±0.5 | 4.0±1.4 | 11.0±1.6 | 4.9±3.7 |
| C(1–21)W3 | 3.7±0.3 | 4.4±1.4 | 6.2±3.5 | 1.8±0.2 | 3.0±0.2 | 3.5±0.4 | 15.7±3.4 | 2.1±0.6 |
| C(1–21)Y3 | 9.1±6.6 | 12.6±2.4* | 13.7±2.0 | 6.4±1.2 | 2.5±0.5 | 3.3±0.3 | 8.8±2.1 | 3.8±0.7 |
| C(4–21) | 3.5±0.7 | 7.8±0.6 | 10.2±3.0 | 3.0±0.3 | 2.1±0.5 | 3.8±1.0 | 10.8±1.1 | 8.1±2.2 |
| C(4–21)W2 | 5.6±1.4 | 7.3±1.3 | 10.1±1.3 | 3.1±1.0 | 1.7±0.6 | 2.5±0.2 | 7.7±1.1 | 3.9±0.5 |
| C(4–21)Y2 | 7.4±4.1 | 11.6±0.7 | 12.4±3.0 | 6.7±1.0 | 2.1±0.3 | 4.2±0.5 | 6.5±0.8 | 4.6±0.8 |
| C(7–21) | 4.5±1.1 | 7.3±0.8 | 8.3±0.9 | 3.1±1.1 | 1.5±0.7 | 4.7±0.1 | 6.5±0.2 | 4.7±1.0 |
| C(7–21)W | 4.1±0.5 | 4.8±1.0 | 6.6±2.7 | 5.5±0.9 | 0.8±0.1 | 2.7±0.3 | 3.4±0.6 | 1.6±0.5 |
| C(7–21)Y | 7.7±4.1 | 9.4±3.9 | 11.6±4.5 | 5.1±1.5 | 1.8±0.1 | 4.3±0.3 | 6.3±0.2 | 5.4±0.2 |
| C(10–21) | 2.5±0.9 | 0.9±0.1 | 1.2±0.4 | 2.0±1.1 | 0.8±0.2 | 1.0±0.1 | 1.4±0.2 | 1.8±0.6 |
| C(10–21)W | 2.6±0.7 | 2.5±0.3 | 3.9±1.4 | 2.1±1.4 | 0.8±0.2 | 1.2±0.0 | 1.5±0.1 | 1.0±0.2 |
| C(10–21)Y | 1.8±0.1 | 0.9±0.1 | 1.4±0.5 | 1.3±2.0 | 0.7±0.5 | 1.1±0.1 | 1.4±0.2 | 0.7±0.2 |
Comparison of peptide-induced gene expression levels relative to that of basal expression levels in the absence of peptide. Amino acid substitutions: W3, [F2W, F5W, F12W]; W2, [F5W, F12W]; W, [F12W]; Y3, [F2Y, F5Y, F12Y]; Y2, [F5Y, F12Y]; Y, [F12Y].
Significance level: *, p<0.05.