Fig 3. Systemic degradation of NETs attenuated organ damage during LPS-induced endotoxic shock.
Endotoxic shock was induced by LPS injection (15 mg/kg, iv.) Mice were pre-treated (10 min) and post-treated (8 h) with saline or rhDNase (10 mg/kg, sc.). Twelve hours after endotoxic shock induction, the serum concentrations of cf-DNA (A) and TNF-α (B), serum levels of serum CK-MB (C), BUN (D), AST (E) and MPO in lung tissue (F) were determined. The data are reported as the mean ± SEM. * p < 0.05 compared with the sham group; # p < 0.05 compared with the LPS+Sal group (ANOVA followed by Tukey’s test, n = 5 per experimental group). (G) Survival rates of endotoxemic mice pre-treated (10 min) and post-treated (8/8 h) with saline or rhDNase (10 mg/kg, sc.) until the 48th hour. * p < 0.05 compared with the LPS+Sal group (Mantel-Cox log-rank test, n = 10 per experimental group).