Fig 2. Salbutamol and terbutaline enhanced MKP-1 expression in J774 macrophages in a dose-dependent manner.

(A and B) J774 macrophages were treated with increasing concentrations of salbutamol or terbutaline in the absence or in the presence of LPS (10 ng/ml) for 1 h. MKP-1 mRNA was measured by quantitative RT-PCR, and MKP-1 mRNA expression levels were normalized against GAPDH mRNA levels. (C) J774 cells were incubated with LPS (10 ng/ml) and salbutamol (100 nM) for 1 hour and MKP-1 protein was measured by Western blot. The chemiluminescent signal was quantified, and the amounts of MKP-1 were normalized against actin. Results are expressed as mean ± S.E.M., n = 6 (A and B) or n = 4 (C). One-way ANOVA with Dunnett´s (A and B) or Bonferroni´s (C) post test was performed, and statistical significance is indicated with * p < 0.05, ** p < 0.01 and *** p < 0.001 as compared to control or LPS-treated cells.