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. 2016 Feb 5;11(2):e0148144. doi: 10.1371/journal.pone.0148144

Fig 4. Salbutamol, terbutaline, 8-Br-cAMP and the p38 MAPK inhibitor BIRB 769 inhibited TNF production in J774 macrophages.

Fig 4

J774 macrophages were stimulated with LPS (10 ng/ml) in the absence or in the presence of salbutamol (A, C and E), terbutaline (B, C and E), 8-Br-cAMP (C and D), rolipram (E) or the p38 MAPK inhibitor BIRB 769 (F) for 24 h (A, B, D, E and F; TNF protein) or for 4 h (C; TNF mRNA). TNF mRNA was measured by quantitative RT-PCR and TNF mRNA expression levels were normalized against GAPDH mRNA levels. TNF protein accumulation in the culture medium was measured by ELISA. Results are expressed as mean ± S.E.M., n = 8 (A-C) or n = 4 (D, E and F). One-way ANOVA with Dunnett´s post test (A and B) or Bonferroni´s post test (C-F) was performed and statistical significance is indicated with * p < 0.05, ** p < 0.01 and *** p < 0.001 as compared to LPS-treated cells. In the Panel 4E, statistical significance (a) is p < 0.001 between LPS+ salbutamol and LPS+ salbutamol+ rolipram treated cells, and (b) is p < 0.001 between LPS+ terbutaline and LPS+ terbutaline+ rolipram treated cells.