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. 2016 Feb 5;11(2):e0148478. doi: 10.1371/journal.pone.0148478

Fig 3. c-di-GMP impacts the SHAPE reactivity of specific regions in Vc1 RNA.

Fig 3

Vc1 RNA transcripts were incubated with or without 500 μM c-di-GMP and analyzed by SHAPE. SHAPE reactivity values for each nucleotide position were obtained by averaging values from five independent experiments. The inset shows in red text the location of the nucleotides with significantly altered reactivity, mapped on the predicted structure of Vc1. The boxes denote the G12, A39 and C104 residues. * P < 0.05 by unpaired t-test comparing SHAPE reactivity values for each nucleotide position, with or without c-di-GMP, indicating significant changes in reactivity in response to c-di-GMP.