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. 2016 Feb 5;11(2):e0148572. doi: 10.1371/journal.pone.0148572

Fig 3. Hypersensitivity of transgenic plants overexpressing NHL6.

Fig 3

(A, B) RT-PCR and qRT-PCR analyses of NHL6 expression level in Col-0, nhl6 and 35S::NHL6 overexpressor lines 3 and 6. ACTIN2 was used as a reference gene for normalisation of expression and the transcript level in Col-0 was set as 1.0. Error bars represent SD (n = 3). (C, D) ABA-mediated inhibition of seed germination greening. Seeds of Col-0, nhl6 and 35S::NHL6 overexpressor lines 3 and 6 were vernalized at 4°C for three days on MS medium, and then transferred to a growth chamber. Photographs were taken after one week. Scale bar = 5 mm. (E) Germination rates on MS medium supplemented with different concentrations of ABA for 2 days. (F) germination time course on MS medium supplemented with 1 μM ABA. Col-0, closed circles; nhl6, open squares, 35S::NHL6-3, open rhombuses; 35S::NHL6-6, closed triangles. Results are presented as average values and standard errors from three experiments. At each time, at least 100 seeds were counted.