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. 2016 Feb 5;11(2):e0148572. doi: 10.1371/journal.pone.0148572

Fig 6. AREBs and ABF3 activated the expression of NHL6.

Fig 6

(A) NHL6 mRNA levels in various ABA signal deficient mutants. Two-week-old seedlings grown on MS medium were treated with 100 μM ABA for 6 hours. NHL6 expression was examined by qRT-PCR analyses after the treatments. ACTIN2 was used as a reference gene for normalisation of expression and the transcript level in the untreated Col-0 seedlings was set as 1.0. Error bars represent SD (n = 3). (B) ABA induced NHL6 expression was significantly blocked in the areb1areb2abf3 triple mutant (base on the published data by Yoshida et al. 2010). (C) Schematic diagram of the vector structure of effectors and reporter used in transient assays. (D) Transient assays of ProNHL6::LUC expression. 35S::REN-NHL6::LUC reporter construct was transiently expressed in Arabidopsis protoplasts together with the control vector, 35S::AREB1, 35S::AREB2, 35S::ABF3 or 35S::ABI5 effectors, respectively. The expression level of REN was used as an internal control. LUC/REN ratio represents the relative activity of the NHL6 promoter. Data are values of three independent experiments. Asterisks indicate significant differences from the corresponding control values at *0.01 < P < 0.05 and **P < 0.01 using the Student’s t-test. (n = 3).