(A) When the ITI is prolonged, the estimated dwelling factor does not decrease significantly in CantonS flies during the first 4s. However, the attention span lasts less than 5s. In the mutant rsh1 flies show wild-type like dwelling for an ITI of 1s, but the attention span lasts less than 3s. The same results are obtained, if the selection procedure (see Methods) for unbiased flies is not applied to the data. (B) For all four sets of CantonS experiments (ITI: 1, 3, 4 and 5s) the response polarity distribution is balanced for left and right responses. In all sets flies flew robustly and produced comparable patterns of responses. It is thus unlikely that differences in the behaviour of different batches of flies cause the observed differences.