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. 2016 Feb 5;11(2):e0148365. doi: 10.1371/journal.pone.0148365

Fig 3. SftA is not required for rescue of spore formation by spoIIIEΔγ intragenic suppressor mutations.

Fig 3

Strains were induced to sporulate for 24–36 h in DSM medium, unsporulated cells were eliminated by heat-kill, and the number of spores was measured by plating for cfu. Sporulation levels are similar for sftA+ and ΔsftA strains bearing spoIIIEΔγ mutant alleles: P260L (BOSE2286; BOSE2492), E312A (BOSE2121; BOSE2486), Y316D (BOSE2284; BOSE2490), P319S (BOSE2321; BOSE2496), A343V (BOSE2288; BOSE2494), E347G (BOSE2323; BOSE2498), P492Q (BOSE2120; BOSE2935), and T617A (BOSE2123; BOSE2488). Sporulation efficiencies for strains harboring full-length (“f.l.”) spoIIIE (bKM776) and spoIIIEΔγ (BOSE2042) are plotted for comparison. The average of at least 3 replicates is plotted. Error bars indicate one standard deviation in each direction.