Fig 6. Mutations that rescue the sporulation defect of spoIIIEΔγ strains do not rescue a vegetative translocation defect.
Growth of strains in various concentrations of the replication-stress-inducing antibiotic novobiocin (nov) was evaluated. Cells were grown to mid-exponential phase in LB, and then diluted to OD600 0.0125 in LB containing 960 ng ml-1 nov (A), 480 ng ml-1 (B), or no nov (C). OD600 measurements are plotted versus hours after dilution. All strains harbor ΔspoIIIE::neo. As indicated, samples were from strains with no ectopic spoIIIE (bDR1066), spoIIIE (bKM776), spoIIIEΔγ (BOSE2042), or a spoIIIEΔγ mutant: P260L (BOSE2286), E312A (BOSE2121), Y316D (BOSE2284), A343V (BOSE2288), E347G (BOSE2323), P492Q (BOSE2120), T617A (BOSE2123). Representative data from one of at least two replicates are plotted.