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. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Nat Biotechnol. 2016 Jan 18;34(2):184–191. doi: 10.1038/nbt.3437

Figure 2.

Figure 2

HPRT1 and NUDT5 confer 6-thioguanine resistance. (a) For each of 6 sgRNAs targeting these genes, fold-enrichment for the indicated sgRNA after two weeks of selection with 6-thioguanine, relative to its starting abundance, assayed in three different cell lines. (b) TIDE analysis of indels for sgRNAs number 4 and 6 from (a) targeting NUDT5 tested in three cell lines. * indicates a sample where no cells survived and thus TIDE analysis could not be performed (c) Schematic of purine metabolism. Proteins are shown in blue circles, small molecules in italics. PRPS1 is also known as PRPP synthetase; PRPP is phosphoribosyl pyrophosphate.