Identified and validated analytes by Luminex (MM). In comparison to control samples, BDNF, ApoE and VCAM-1 were significantly elevated in all NCLs samples (a,c,e). When comparing only JNCL and control samples, BDNF and ApoE were significantly elevated; VCAM-1 was not significantly different (b,d,f). Bars indicate mean ± SEM. Statistical significance determined using Student’s t test for all analytes except BDNF, determined by Mann-Whitney U test (***, P < 0.0001; *, 0.002 ≤ P ≤ 0.05).