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Micrographs of the cholesterol depletion process. Heart-shaped regions (gray) are individual supported lipid bilayers formed from the rupture of giant unilamellar vesicles onto a glass coverslip (black). mβCD is added at t = 0 s. mβCD removes cholesterol from the supported lipid bilayers. Large holes form in the bilayers as their areas decrease, further revealing the glass coverslip beneath them. DMPC/cholesterol bilayers are shown here, [mβCD] = 2.5 mM.
