Table 1.
Previously Published Measurements of Cholesterol Accessibility for Two-Component PC-Lipid Bilayers
| PC-Lipida | Characteristic χCb | Assay | Reference |
|---|---|---|---|
| di-16:0 | 0.63, 0.58 | COD | (18) |
| 0.50 | COD | (16) | |
| di-14:0 | 0.50 | COD | (16) |
| 18:0-18:1 | 0.50 | COD | (16) |
| 16:0-18:1 | 0.58, 0.52, 0.40, 0.25 | COD | (18) |
| 0.50 | COD | (16) | |
| 0.334, 0.250 | COD | (17)c | |
| di-18:1 | 0.62, 0.57, 0.51, 0.40, 0.25 | COD | (18) |
| 0.33 | COD | (16) | |
| di-18:0 | 0.47d | PFO | (11) |
| di-16:0 | 0.49d | PFO | (11) |
| di-14:0 | 0.51d | PFO | (11) |
| 18:0-18:1 | 0.42d | PFO | (11) |
| 16:0-18:1 | 0.47d | PFO | (14) |
| 0.45d | PFO | (14) | |
| 0.45 | PFO | (12) | |
| 0.44d | PFO | (11) | |
| 0.44d | PFO | (13) | |
| 0.28d | PFO, pH = 7.4 | (10) | |
| 0.24d | PFO, pH = 5.1 | (10) | |
| di-18:1 | 0.45 | PFO-D4e | (15) |
| 0.45 | ALO-D4e | (15) | |
| 0.41 | PFO | (15) | |
| 0.37d | PFO | (11) | |
| 0.35 | PFO | (12) | |
| 0.34d | PFO | (14) | |
| 0.26 | ALO | (15) | |
| 0.26d | PFO, pH = 7.4 | (10) | |
| 0.20d | PFO, pH = 7.0 | (10) | |
| di-(4Me-16:0) | 0.31 | PFO | (15) |
| 0.27 | PFO-D4e | (15) | |
| 0.27 | ALO-D4e | (15) | |
| 0.25 | PFO | (12) | |
COD, cholesterol oxidase; PFO, perfringolysin O; ALO, anthrolysin O.
a
Lipids are listed by the number of carbons and degree of unsaturation of their tails (e.g., DMPC is di-14:0-PC).
b
Characteristic χC refers to the one or several mole fraction(s) of cholesterol at or above which the accessibility of cholesterol increases sharply. Only characteristic χC ≥ 0.20 are listed.
c
The researchers did not report on bilayers with χC > 0.345.
d
This value was interpreted from a graph as the half-maximum of cytolysin binding.
e
This version of the protein is monomeric and does not cause pores to form in the membrane.