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. 2016 Feb 2;110(3):612–622. doi: 10.1016/j.bpj.2015.12.019

Figure 2.

Figure 2

Biophysical behavior of POPC/Chol/C16-GlcCer mixtures containing a constant POPC composition. (A–F) t-PnA (A and D) fluorescence anisotropy, (B and E) mean fluorescence lifetime, and (C and F) long-lifetime component of t-PnA intensity decay in ternary POPC/Chol/C16-GlcCer mixtures, as a function of (A–C) the C16-GlcCer molar fraction or (D–F) the C16-GlcCer/Chol ratio. The mixtures contain 40 (triangles) or 70 (circles) mol % of POPC. Measurements were performed at pH 7.4 (solid symbols) and pH 5.5 (open symbols). Values are means ± SD of at least three independent experiments.