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. 2016 Feb 2;110(3):612–622. doi: 10.1016/j.bpj.2015.12.019

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© 2016 by the Biophysical Society.

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Biophysical characterization of POPC/Chol/C16-GlcCer mixtures with a constant POPC/Chol ratio. (A–F) t-PnA (A and B) fluorescence anisotropy, (C and D) mean fluorescence lifetime, and (E and F) long-lifetime component of the intensity decay in ternary POPC/Chol/C16-GlcCer mixtures with POPC/Chol ratios mimicking 25 (black circles) and 75 (gray circles) mol % of an l_o phase. Measurements were performed at (A, C, and E) neutral and (B, D, and F) acidic pH. Values are means ± SD of at least three independent experiments.